منابع مشابه
Cell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobody
Objective(s): Filamentous bacteriophage M13 was genetically engineered to specifically target mammalian cells for gene delivery purpose. Materials and Methods: A vascular endothelial growth factor receptor 2 (VEGFR2)-specific nanobody was genetically fused to the capsid gene III of M13 bacteriophage (pHEN4/3VGR19). A mammalian expression construct containing Cop-green fluorescent protein (Cop-G...
متن کاملThe Proteins of Bacteriophage M13 by Timothy J. Henry and David Pratt
-Particles of the small filamentous coliphage M13 contain not only the major coat protein, which is the product of phage gene 8, but also a minor coat protein, the A protein, which is the product of gene 3. The A protein has a molecular weight of approximately 70,000 daltons, is present in one copy per virion, and is responsible for phage attachment to host cells. Also associated with purified ...
متن کاملConformations of the single-stranded DNA of bacteriophage M13.
At least two conformations of M13 single-stranded DNA have been demonstrated by measuring differences in sedimentation coefficient and by direct visualization in the electron microscope. Which form is obtained from infected cells and/or intact phage depends on the pH, ionic strength, and temperature. The slower-sedimenting form can be converted to the faster-sedimenting, single-stranded form by...
متن کاملIllegitimate recombination at the replication origin of bacteriophage M13.
Hybrids composed of phage M13 and plasmid pHV33 were used to study the formation of deletions in Escherichia coli. Eighty to ninety percent of the deletion endpoints were at the position of the nick introduced into the M13 replication origin by the phage gene II protein. This suggests the existence of a novel mechanism of illegitimate recombination.
متن کاملFrom the Cover: Single M13 bacteriophage tethering and stretching
www.pnas.org/cgi/content/full/104/12/4892#otherarticles This article has been cited by other articles: E-mail Alerts . click here at the top right corner of the article or Receive free email alerts when new articles cite this article sign up in the box Rights & Permissions www.pnas.org/misc/rightperm.shtml To reproduce this article in part (figures, tables) or in entirety, see: Reprints www.pna...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1969
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.62.3.800